Journal: Clinical and Translational Radiation Oncology

Article Title: MonoHER selectively enhances the radiotherapy response in p53 wild-type breast cancer via stabilization of p53

doi: 10.1016/j.ctro.2026.101147

Figure Lengend Snippet: Different protein expression of p-ATM, p- p 53 and p53 in breast cancer cells treated with combination of monoHER (M) and radiation (RT). Representative Western blots of p-ATM, p-p53 and p53 in MCF7 (A), T47D (E) and MCF10A (H) cells. Quantification of p-ATM (B), p-p53 (C) and p53 (D) protein expression in MCF7 cells. Quantification of p-ATM (E), p-p53 (F) and p53 (G) protein expression in T47D cells. Quantification of p-ATM (I), p-p53 (J) and p53 (K) protein expression in MCF10A cells. Data are presented as mean ± SEM from three independent experiments. *p < 0.05, **p < 0.01.

Article Snippet: Membranes were blocked in 5% non-fat milk in TBST for 1 h at RT and incubated overnight at 4°C with primary antibodies against p-ATM (1:2000; Cell Signaling Technology, 5883), p-p53 (1:1000; Cell Signaling Technology, 9284) and p53 (1:1000; Cell Signaling Technology, 9282). α-Tubulin (1:1000, Sigma Aldrich, T7451) or Vinculin (1:1000, Sigma Aldrich, V9131) were used as loading controls.

Techniques: Expressing, Western Blot

Journal: Clinical and Translational Radiation Oncology

Article Title: MonoHER selectively enhances the radiotherapy response in p53 wild-type breast cancer via stabilization of p53

doi: 10.1016/j.ctro.2026.101147

Figure Lengend Snippet: Monoher interacts with p53. Biomolecular interactions of monoHER with wild-type p53 (A) and mutant p53 (B). Docking scores are indicated at the bottom of the image. Representative Western blots and quantification (E) of the CETSA experiment with cell lysates of MCF7 cells, which were treated with DMEM (C) or monoHER (D). Data are presented as mean ± SEM from three independent experiments.

Article Snippet: Membranes were blocked in 5% non-fat milk in TBST for 1 h at RT and incubated overnight at 4°C with primary antibodies against p-ATM (1:2000; Cell Signaling Technology, 5883), p-p53 (1:1000; Cell Signaling Technology, 9284) and p53 (1:1000; Cell Signaling Technology, 9282). α-Tubulin (1:1000, Sigma Aldrich, T7451) or Vinculin (1:1000, Sigma Aldrich, V9131) were used as loading controls.

Techniques: Mutagenesis, Western Blot

Journal: iScience

Article Title: SKA2 promotes gastric cancer progression by regulating glutathione metabolism

doi: 10.1016/j.isci.2026.115202

Figure Lengend Snippet: Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.

Article Snippet: p-ATM , Cell Signaling Technology , Cat# 5883; RRID: AB_10835213.

Techniques: Western Blot, Expressing, Knockdown, Over Expression, Control